REDS-IV-P Publications

Publications

The Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P): A research program striving to improve blood donor safety and optimize transfusion outcomes across the lifespan

Josephson CD, Glynn S, Mathew S, Birch R, Bakkour S, Baumann Kreuziger L, Busch MP, Chapman K, Dinardo C, Hendrickson J, Hod EA, Kelly S, Luban N, Mast A, Norris P, Custer B, Sabino E, Sachais B, Spencer BR, Stone M, Kleinman S; National Heart, Lung, and Blood Institute (NHLBI) Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P). The Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P): A research program striving to improve blood donor safety and optimize transfusion outcomes across the lifespan. Transfusion. 2022 Apr 19. doi: 10.1111/trf.16869. Epub ahead of print. PMID: 35441384.

BloodBank Guy Podcast 098CE: “Simply REDS” with Steve Kleinman & Cassandra Josephson

Chaffin, J. (Host). (2022, July 18). 098CE: “Simply REDS” with Steve Kleinman & Cassandra Josephson [Audio podcast episode]. In Blood Bank Guy Essentials Podcast.Blood Bank Guy.https://www.bbguy.org/2022/07/18/098/

How do we build a comprehensive Vein-to-Vein (V2V) database for conduct of observational studies in transfusion medicine? Demonstrated with the Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric V2V database protocol

Birch RJ, Umbel K, Karafin MS, Goel R, Mathew S, Pace W; NHLBI Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P). How do we build a comprehensive Vein-to-Vein (V2V) database for conduct of observational studies in transfusion medicine? Demonstrated with the Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric V2V database protocol. Transfusion. 2023 Sep;63(9):1623-1632. doi: 10.1111/trf.17507. Epub 2023 Aug 19. PMID: 37596918.

Recall Donors

Samples originally part of the REDS-III RBC-Omics study.

The following publications were partially funded by REDS-IV-P or used REDS-IV-P resources:

Sphingosine 1-phosphate has a negative effect on RBC storage quality

Hay A, Nemkov T, Gamboni F, Dzieciatkowska M, Key A, Galbraith M, Bartsch K, Sun K, Xia Y, Stone M, Busch MP, Norris PJ, Zimring JC, D’Alessandro A. Sphingosine 1-phosphate has a negative effect on RBC storage quality. Blood Adv. 2023 Apr 25;7(8):1379-1393. doi: 10.1182/bloodadvances.2022008936. PMID: 36469038; PMCID: PMC10139937.

Regulation of kynurenine metabolism by blood donor genetics and biology impacts red cell hemolysis in vitro and in vivo

Nemkov T, Stephenson D, Erickson C, Dzieciatkowska M, Key A, Moore A, Earley EJ, Page GP, Lacroix IS, Stone M, Deng X, Raife TJ, Kleinman SH, Zimring JC, Roubinian NH, Hansen KC, Busch MP, Norris PJ, D’Alessandro A. Regulation of kynurenine metabolism by blood donor genetics and biology impacts red cell hemolysis in vitro and in vivo. Blood. 2023 Nov 17:blood.2023022052. doi: 10.1182/blood.2023022052. Epub ahead of print. PMID: 37976448.

Genetic polymorphisms and expression of Rhesus blood group RHCE are associated with 2,3-bisphosphoglycerate in humans at high altitude

D’Alessandro A, Earley EJ, Nemkov T, Stephenson D, Dzieciatkowska M, Hansen KC, Minetti G, Champigneulle B, Stauffer E, Pichon A, Furian M, Verges S, Kleinman S, Norris PJ, Busch MP, Page GP, Kaestner L. Genetic polymorphisms and expression of Rhesus blood group RHCE are associated with 2,3-bisphosphoglycerate in humans at high altitude. Proc Natl Acad Sci U S A. 2024 Jan 2;121(1):e2315930120. doi: 10.1073/pnas.2315930120. Epub 2023 Dec 26. PMID: 38147558.

RESPONSE

SARS-CoV-2 seroprevalence and neutralizing activity in donor and patient blood from the San Francisco Bay Area

Ng DL, Goldgof GM, Shy BR, Levine AG, Balcerek J, Bapat SP, Prostko J, Rodgers M, Coller K, Pearce S, Franz S, Du L, Stone M, Pillai SK, Sotomayor-Gonzalez A, Servellita V, Martin CSS, Granados A, Glasner DR, Han LM, Truong K, Akagi N, Nguyen DN, Neumann NM, Qazi D, Hsu E, Gu W, Santos YA, Custer B, Green V, Williamson P, Hills NK, Lu CM, Whitman JD, Stramer S, Wang C, Reyes K, Hakim JMC, Sujishi K, Alazzeh F, Pham L, Oon CY, Miller S, Kurtz T, Hackett J Jr, Simmons G, Busch MP, Chiu CY. SARS-CoV-2 seroprevalence and neutralizing activity in donor and patient blood from the San Francisco Bay Area. medRxiv [Preprint]. 2020 May 25:2020.05.19.20107482. doi: 10.1101/2020.05.19.20107482. Update in: Nat Commun. 2020 Sep 17;11(1):4698. PMID: 32511477; PMCID: PMC7273245.

Minipool testing for SARS-CoV-2 RNA in United States blood donors

Bakkour S, Saá P, Groves JA, Montalvo L, Di Germanio C, Best SM, Grebe E, Livezey K, Linnen JM, Strauss D, Kessler D, Bonn M, Green V, Williamson P, Kleinman S, Stramer SL, Stone M, Busch MP; NHLBI Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P). Minipool testing for SARS-CoV-2 RNA in United States blood donors. Transfusion. 2021 May 27. doi: 10.1111/trf.16511. Epub ahead of print. PMID: 34046906.

Use of U.S. Blood Donors for National Serosurveillance of SARS-CoV-2 Antibodies: Basis for an Expanded National Donor Serosurveillance Program

Stone M, Di Germanio C, Wright DJ, Sulaeman H, Dave H, Fink RV, Notari EP, Green V, Strauss D, Kessler D, Destree M, Saa P, Williamson PC, Simmons G, Stramer SL, Opsomer J, Jones JM, Kleinman S, Busch MP, NHLBI Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P). Use of U.S. Blood Donors for National Serosurveillance of SARS-CoV-2 Antibodies: Basis for an Expanded National Donor Serosurveillance Program. Clinical Infectious Diseases. 2021 June 10. doi: 10.1093/cid/ciab537.

Frequent detection but lack of infectivity of SARS-CoV-2 RNA in presymptomatic, infected blood donor plasma

Saá P, Fink RV, Bakkour S, Jin J, Simmons G, Muench MO, Dawar H, Di Germanio C, Hui AJ, Wright DJ, Krysztof DE, Kleinman SH, Cheung A, Nester T, Kessler DA, Townsend RL, Spencer BR, Kamel H, Vannoy JM, Dave H, Busch MP, Stramer SL, Stone M, Jackman RP, Norris PJ; NHLBI Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P). Frequent detection but lack of infectivity of SARS-CoV-2 RNA in presymptomatic, infected blood donor plasma. J Clin Invest. 2022 Sep 1;132(17):e159876. doi: 10.1172/JCI159876. PMID: 35834347; PMCID: PMC9435642.

Prevalence of SARS-CoV-2 Viremia in Presymptomatic Blood Donors in the Delta and Omicron Variant Eras

Saá P, Fink RV, Dawar H, Di Germanio C, Montalvo L, Wright DJ, Krysztof DE, Kleinman SH, Nester T, Kessler DA, Townsend RL, Spencer BR, Kamel H, Vannoy J, Busch MP, Stramer SL, Stone M, Norris PJ; NHLBI Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P). Prevalence of SARS-CoV-2 Viremia in Presymptomatic Blood Donors in the Delta and Omicron Variant Eras. Open Forum Infect Dis. 2023 May 9;10(5):ofad253. doi: 10.1093/ofid/ofad253. PMID: 37250174; PMCID: PMC10220507.

Clinical outcomes in hospitalized plasma and platelet transfusion recipients prior to and following widespread blood donor SARS-CoV-2 infection and vaccination

Roubinian NH, Greene J, Liu VX, Lee C, Mark DG, Vinson DR, Spencer BR, Bruhn R, Bravo M, Stone M, Custer B, Kleinman S, Busch MP, Norris PJ; NHLBI Recipient Epidemiology and Donor Evaluation Study-IV-P (REDS-IV). Clinical outcomes in hospitalized plasma and platelet transfusion recipients prior to and following widespread blood donor SARS-CoV-2 infection and vaccination. Transfusion. 2023 Dec 6. doi: 10.1111/trf.17616. Epub ahead of print. PMID: 38054619.


Database analyses

Variation in Neonatal Transfusion Practice

Patel RM, Hendrickson JE, Nellis ME, Birch R, Goel R, Karam O, Karafin MS, Hanson SJ, Sachais BS, Hauser RG, Luban NLC, Gottschall J, Josephson CD, Sola-Visner M; National Heart, Lung, and Blood Institute Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P). Variation in Neonatal Transfusion Practice. J Pediatr. 2021 Apr 7:S0022-3476(21)00323-1. doi: 10.1016/j.jpeds.2021.04.002. Epub ahead of print. PMID: 33836184.

Transfusion Practices in a Large Cohort of Hospitalized Children

Nellis ME, Goel R, Hendrickson JE, Birch R, Patel RM, Karafin MS, Hanson SJ, Sachais BS, Hauser RG, Luban NLC, Gottschall J, Sola-Visner M, Josephson CD, Karam O; NHLBI Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P). Transfusion Practices in a Large Cohort of Hospitalized Children. Transfusion. 2021 May 11. doi: 10.1111/trf.16443. Epub ahead of print. PMID: 33973660.
[Selected for Transfusion continuing medical education (CME)

Transfusion Practices in Pediatric Cardiac Surgery Requiring Cardiopulmonary Bypass: A Secondary Analysis of a Clinical Database

Hanson SJ, Karam O, Birch R, Goel R, Patel RM, Sola-Visner M, Sachais BS, Hauser RG, Luban NLC, Gottschall J, Josephson CD, Hendrickson JE, Karafin MS, Nellis ME; NHLBI Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P). Transfusion Practices in Pediatric Cardiac Surgery Requiring Cardiopulmonary Bypass: A Secondary Analysis of a Clinical Database. Pediatr Crit Care Med. 2021 Jul 14. doi: 10.1097/PCC.0000000000002805. Epub ahead of print. PMID: 34261944.

Transfusion Practices for Pediatric Oncology and Hematopoietic Stem Cell Transplantation Patients: Data from the National Heart Lung and Blood Institute Recipient Epidemiology and Donor Evaluation Study-III (REDS-III)

Goel R, Nellis ME, Karam O, Hanson SJ, Tormey CA, Patel RM, Birch R, Sachais BS, Sola-Visner MC, Hauser RG, Luban NLC, Gottschall J, Josephson CD, Hendrickson JE, Karafin MS; NHLBI Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P). Transfusion practices for pediatric oncology and hematopoietic stem cell transplantation patients: Data from the National Heart Lung and Blood Institute Recipient Epidemiology and Donor Evaluation Study-III (REDS-III). Transfusion. 2021 Sep;61(9):2589-2600. doi: 10.1111/trf.16626. Epub 2021 Aug 29. PMID: 34455598.

Donor genetic and non-genetic factors affecting red blood cell transfusion effectiveness

Roubinian NH, Reese SE, Qiao H, Plimier C, Fang F, Page GP, Cable RG, Custer B, Gladwin MT, Goel R, Harris B, Hendrickson JE, Kanias T, Kleinman S, Mast AE, Sloan SR, Spencer BR, Spitalnik SL, Busch MP, Hod EA. Donor genetic and non-genetic factors affecting red blood cell transfusion effectiveness. JCI Insight. 2021 Nov 18:e152598. doi: 10.1172/jci.insight.152598. Epub ahead of print. PMID: 34793330.

Associations between ABO non-identical platelet transfusions and patient outcomes-A multicenter retrospective analysis

Bougie DW, Reese SE, Birch RJ, Bookwalter DB, Mitchell PK, Roh D, Kreuziger LB, Cable RG, Goel R, Gottschall J, Hauser RG, Hendrickson JE, Hod EA, Josephson CD, Kahn S, Kleinman SH, Mast AE, Ness PM, Roubinian NH, Sloan S; NHLBI Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P). Associations between ABO non-identical platelet transfusions and patient outcomes-A multicenter retrospective analysis. Transfusion. 2023 Mar 30. doi: 10.1111/trf.17319. Epub ahead of print. PMID: 36994786.


Abstract Presentations – American Association of Blood Banks (AABB)

Annual Meeting 2020

Variation in Neonatal Transfusion Practice in the United States: Data from the NHLBI Recipient Epidemiology and Donor Evaluation Study III (REDS-III)

R M Patel, M Sola-Visner, M E Nellis, R Birch, R Goel, O Karam, M S Karafin, S J Hanson, B S Sachais, R G Hauser, N LC Luban, J L Gottschall, C D Josephson, and J E Hendrickson; for the NHLBI Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P).

Associations of blood donor polygenetic risk for osmotic hemolysis and hemoglobin increments following red blood cell transfusion

N Roubinian, S E Reese, H Qiao, T Kanias, G Page, R Cable, B Custer, S Kleinman, M Busch, E Hod; for the NHLBI Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P).

Pediatric Transfusion Practice in the United States: Data from the NHLBI Recipient Epidemiology and Donor Evaluation Study III (REDS-III)

M E Nellis, R Goel, S J Hanson, R Birch, M S Karafin, B S Sachais, R G Hauser, R M Patel, M Sola-Visner, N LC Luban, J L Gottschall, C D Josephson, J E Hendrickson, and O Karam; for the NHLBI Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P).

Analysis of Electronic Health Record Data: Quality control issues to consider in linking donor, component, and recipient data

S E Reese, R Birch, N Roubinian, E Hod, H Qiao, K Khan, R V Salgado, B Harris, and S Mathew

Clinical and Genetic Predictors of Red Blood Cell Alloimmunization in a Brazilian Sickle Cell Disease Cohort

S Kelley, F Fang, D Teles, C Dinardo, M do Carmo Valguiero, L Cayres, S Lopes de Castilho, E Sabino, G Page, B Custer; for the NHLBI Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P).

Minipool Testing for SARS-CoV-2 RNA in US Blood Donors

S Bakkour, M Stone, C Di Germanio, K Livezey, J M Linnen, P Saá, S L Stramer, V Green, P Williamson, D Strauss, D Kessler, M Destree, S Kleinman, M P Busch; for the NHLBI Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P).

Blood Donor Surveillance of SARS-CoV-2 Seroreactivity

M Stone, C Di Germanio, E Notari, H Sulaeman, V Green, P Williamson, D Strauss, D Kessler, M Destree, SL Stramer, S Kleinman, MP Busch; for the NHLBI Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P).

Big Data and Large Randomized Controlled Trials Shaping Transfusion Practices in Little People

C D Josephson

Annual Meeting 2021

Transfusion Practice in Pediatric Spinal Fusion Surgeries

M E Nellis, R Birch, N Luban; for the NHLBI Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P).

Optimization of red blood cell biotinylation protocol produced by a centralized laboratory

T Kanias; for the NHLBI Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P).

Sars-Cov-2 RNA Detection in Plasma from Blood Donors with Symptoms or Diagnosis Postdonation

S Bakkour; for the NHLBI Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P).

Trends in PDI symptom reporting before and after start of COVID pandemic

P Saá; for the NHLBI Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P).

Annual Meeting 2022

Multi-center Evaluation of ABO Mismatched Platelet Transfusions on Mortality

D Bougie, S Reese, R Birch, D Bookwalter, P Mitchell; for the NHLBI Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P).

Annual Meeting 2023

REDS Abstract Spotlight

M Busch; for the NHLBI Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P).

Metabolomics analysis of over 13,000 donors from the REDS RBC Omics study identifies markers of hemolysis and in vivo hemoglobin increments

A D’Alessandro; for the NHLBI Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P).

Metabolite levels from end of storage red blood cells are reproducible within the same donor across multiple donations

A D’Alessandro; for the NHLBI Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P).

What’s in a blood bag? Exposome 2.0

A D’Alessandro; for the NHLBI Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P).

Similar rates of SARS-CoV-2 RNAemia in pre-symptomatic blood donors during the delta and omicron variant eras

P Saá; for the NHLBI Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P).

RNA persistence, antibody dynamics and symptom outcomes in a longitudinal cohort of SARS-CoV-2 infection

M Stone; for the NHLBI Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P).

Intensive care unit length of stay and hospital mortality in plasma and platelet transfusion recipients prior to and following periods of blood donor SARS-CoV-2 infection and vaccination

N Roubinian; for the NHLBI Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P).

Abstract Presentations – Pediatric Academic Society (PAS)

Annual Meeting 2021

Variation in Neonatal Transfusion Practice

R M Patel, J E Hendrickson, M E Nellis, R Birch, R Goel, O Karam, M S Karafin, S J Hanson, B S Sachais, R G Hauser, N Luban, J Gottschall, C D Josephson, M Sola-Visner; for the NHLBI Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P).

Annual Meeting 2023

Pre-Transfusion Platelet Counts in Critically Ill Children with Extracorporeal Membrane Oxygenation

O Karam, R Birch; for the NHLBI Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P).

Abstract Presentations – International Society of Blood Transfusion (ISBT)

ISBT 2022 Virtual Congress

Hemovigilance: The US experience from Adult to Pediatric

R Goel, N Luban

ISBT Gothenburg 2023

Chemical individuality of the blood donor as gleaned by high-throughput metabolomics of over 13,000 end of storage red blood cell samples and multi-omics analyses of 643 recalled donors

A D’Alessandro

Trends in thromboses and oxygen requirements in plasma and platelet transfusion recipients prior to and following periods of blood donor SARS-CoV-2 infection and vaccination

N Roubinian, D Wright; for the NHLBI Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P).

Abstract Presentations – American Society of Hematology (ASH)

Annual Meeting 2022

Mirages – REDS Explorer: An Online Portal for Longitudinal Metabolomics Data from the REDS-III RBC Omics Study

A D’Alessandro; for the NHLBI Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P).

Annual Meeting 2023

Genetic Regulation of Carnitine Metabolism Controls Lipid Damage Repair Mechanisms and Hemolytic Propensity of Human Red Blood Cells during Aging In Vivo and in Vitro

A D’Alessandro; for the NHLBI Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P).

Genetic Polymorphisms in the Ferrireductase STEAP3 Regulate a Ferroptosis-like Process of Lipid Peroxidation-Induced Hemolysis in Murine and Human Red Blood Cells

A D’Alessandro; for the NHLBI Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P).

Assessing donor and platelet transfusion characteristics on intracranial hemorrhage outcomes

D Roh; for the NHLBI Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P).

Presentation – BloodNet -Pediatric Intensive Care Meeting

Overview of REDS IV P and Update on Progress

C D Josephson; for the NHLBI Recipient Epidemiology and Donor Evaluation Study-IV-Pediatric (REDS-IV-P).